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Research Laboratory Technician (Fixed Term)



The post holder will be integrated into a research team studying the functional role of the imprinted microRNA-483 in vivo. For this purpose, we have generated loss and gain-of-function mutations in the mouse and are currently analysing their phenotypes in the context of cellular and organismic growth, metabolic function and cancer. The day-to-day job requires helping out with management of mouse colonies (from setting up breeding pairs to plug-checking and ear biopsies to genotyping by PCR), and application of histological techniques for the purpose of immunohistochemistry and stereological measurements. In addition, the post holder will be performing RNA and protein extractions and help out with molecular cloning and luciferase assays.

Essential requirements: HO animal license, experience in mouse husbandry and genotyping by PCR, morphometry/stereological analysis. Basic knowledge of molecular biology techniques (PCR, Q-PCR, Western blotting, immunohistochemistry).

Fixed-term: The funds for this post are available for 6 months in the first instance.

Applications including a CHRIS/5 (http://www.admin.cam.ac.uk/offices/hr/forms/chris5/) and covering letter can emailed to jh483@medschl.cam.ac.uk.

The deadline for applications is 11am 30th September 2014. Those selected for interview will be contacted later that day.

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