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School of Clinical Medicine > Department of Obstetrics & Gynaecology

Group Protocols

CAST newCAST Sterology user guide
Acquisition VIS Acquisition Module user guide

Molecular Biology Protocols

Gel Protocol Running a Gel
Enzyme Digest Protocol for Enzyme Digestion of DNA
DNA Precipitation DNA Precipitation Protocol using Ammonium Acetate
DNA Precipitation DNA Precipitation Protocol using Potassium Acetate
DNA Ligation DNA Ligation Protocol
Beta Actin PCR Beta Actin PCR
Phenol Extraction DNA Extraction Protocol using Phenol
DNA Extraction DNA Extraction Protocol using Phenol & Chloroform
Gel Extraction Gel Extraction Protocol
Transgenic DNA Preparation DNA Preparation for injection into Transgenic animals
Miniprep Qaigen Miniprep Kit Instructions
Midiprep Qaigen Midiprep Kit Instructions
Maxiprep Qaigen Maxiprep Kit Instructions

Immunohistochemistry Protocols

CD31 CD31 Endothelial Cells
CD45 CD45 Leucocytes
F4/80 F4/80 Macrophages
7/4 7/4 Neutrophils
Prot K Proteinase K Epitope Retrieval
Trypsin Trypsin Epitope Retrieval
ABC Troubleshooting ABC Kit Troubleshooting guide
MOM Kit Mouse On Mouse Kit Protocol
MOM Troubleshooting Mouse On Mouse Kit Troubleshooting guide
Blocks Peroxidase & Avidin/Biotin Blocking Solutions
Buffers Washing Buffer Recipes

HUVEC Culture Protocols

Isolation Extraction & Isolation of HUVEC cells
Passage Passage of HUVEC cells
Freezing Freezing of HUVEC cells
Thawing Thawing of HUVEC cells

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Department of Obstetrics & Gynaecology, Level 2, The Rosie Hospital (Addenbrooke's Box 223), Robinson Way, Cambridge CB2 2SW
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